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[One Step]WARS Antibody Kit (RK05722)

Datasheet

ABclonal:

Overview

Product name[One Step]WARS Antibody Kit
Catalog No.RK05722

Product Component

ComponentNameRecommended DilutionApplicationsSpecies Reactivity
Primary AntibodyTryptophanyl-tRNA synthetase 1 Rabbit pAb1:1000 dilutionWBHuman, Mouse, Rat
Secondary AntibodyHRP Goat Anti-Rabbit IgG (H+L)1:10000 dilution
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Tryptophanyl-tRNA synthetase 1 (NP_776049.1).
SequenceMPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFT
Gene ID7453
Swiss protP23381
SynonymsWARS; GAMMA-2; IFI53; IFP53
Calculated MW48kDa/53kDa
Observed MW53-55kDa
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCytoplasm
ABclonal:}

Western blot analysis of extracts of various cells, using WARS antibody (A5758) at 1:1000 dilution ratio through one-step method.

* For research use only. Not for therapeutic or diagnostic purposes.

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