Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Western blot - VCP Rabbit pAb (A13368)
Western blot analysis of extracts of various cell lines, using VCP antibody (A13368) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry - VCP Rabbit pAb (A13368)
Immunohistochemistry analysis of paraffin-embedded human placenta using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - VCP Rabbit pAb (A13368)
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - VCP Rabbit pAb (A13368)
Immunohistochemistry analysis of paraffin-embedded rat kidney using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - VCP Rabbit pAb (A13368)
Immunofluorescence analysis of C6 cells using VCP antibody (A13368) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - VCP Rabbit pAb (A13368)
Immunofluorescence analysis of U-2 OS cells using VCP antibody (A13368) at dilution of 1:100. Blue: DAPI for nuclear staining.