Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

phospho-HCK-Y522 Rabbit pAb (AP0620)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - phospho-HCK-Y522 Rabbit pAb (AP0620)

Western blot analysis of extracts of Jurkat cells, using Phospho-HCK-Y522 antibody (AP0620).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name phospho-HCK-Y522 Rabbit pAb
Catalog No. AP0620
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding Y522 of human HCK
Sequence Email for sequence
Gene ID 3055
Swiss prot P08631
Synonyms JTK9; AIPCV; p59Hck; p61Hck; phospho-HCK-Y522
Calculated MW 60kDa
Observed MW 60kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat
Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Lipid-anchor, Lysosome, Membrane, Nucleus, caveola, cytoskeleton, cytosol, cytosol, focal adhesion, podosome membrane, secretory vesicle

Research Area

phospho-HCK-Y522 Rabbit pAb images

ABclonal:Western blot - phospho-HCK-Y522 Rabbit pAb (AP0620)}

Western blot - phospho-HCK-Y522 Rabbit pAb (AP0620)

Western blot analysis of extracts of Jurkat cells, using Phospho-HCK-Y522 antibody (AP0620).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on HCK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HCK. (Distance between topics and target gene indicate popularity.) HCK

* Data provided by citexs.com, for reference only.

Publishing research using AP0620? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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