Products > Catalog Antibodies > Antibody Duos > Phosphorylated Antibody Duos

Phospho-Stat3-S727 Antibody Kit (RK04089)

Review (1)Publications (0) Datasheet

ABclonal:Western blot - Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Immunoprecipitation - Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Overview

Product namePhospho-Stat3-S727 Antibody Kit
Catalog No.RK04089

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-Stat3-S727 pAbAP0474WBIHCICCIFIPChIPChIPseqRIPFCHuman, Mouse, Rat
[KO Validated] STAT3 Polyclonal AntibodyA1192WBIHCICCIFIPChIPChIPseqRIPFCHuman, Mouse, Rat, Monkey
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic phosphorylated peptide around S727 of human Stat3 (NP_644805.1).
Recombinant fusion protein containing a sequence corresponding to amino acids 640-770 of human STAT3 (NP_644805.1).
SequencePMSPR
Gene ID6774
Swiss protP40763
SynonymsADMIO; ADMIO1; APRF; HIES; ADMIO; APRF; HIES
Calculated MW83kDa/87kDa/88kDa
Observed MW86kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCytoplasm, Nucleus
Phospho-Stat3-S727 pAb (AP0474)
ABclonal:Western blot - Phospho-Stat3-S727 pAb (AP0474) }
Western blot - Phospho-Stat3-S727 pAb (AP0474)

Western blot analysis of extracts of various cell lines, using Phospho-Stat3-S727 antibody (AP0474) at 1:1000 dilution or STAT3 antibody (A1192). NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes or treated by UV at room temperature for 15-30 minutes. C6 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 20 minutes or treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474) }
Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-Stat3-S727 antibody (AP0474) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474) }
Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)

Immunohistochemistry of paraffin-embedded human lung using Phospho-Stat3-S727 antibody (AP0474) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474) }
Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-Stat3-S727 antibody (AP0474) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474) }
Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Stat3-S727 antibody (AP0474) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474) }
Immunohistochemistry - Phospho-Stat3-S727 pAb (AP0474)

Immunohistochemistry of paraffin-embedded mouse lung using Phospho-Stat3-S727 antibody (AP0474) at dilution of 1:100 (40x lens).

ABclonal:Immunoprecipitation - Phospho-Stat3-S727 pAb (AP0474) }
Immunoprecipitation - Phospho-Stat3-S727 pAb (AP0474)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Stat3-S727 pAb (AP0474). Western blot was performed from the immunoprecipitate using Phospho-Stat3-S727 pAb (AP0474) at a dilition of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.


[KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Western blot analysis of extracts of various cell lines, using STAT3 antibody (A1192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (A1192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Immunofluorescence analysis of rat brain using STAT3 antibody (A1192) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192) }
Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Immunofluorescence analysis of mouse brain using STAT3 antibody (A1192) at dilution of 1:100. Blue: DAPI for nuclear staining.

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