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Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody Kit (RK04105)

Reviews (4)Publications (0) Datasheet

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Overview

Product namePhospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody Kit
Catalog No.RK04105

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAbAP0472WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
ERK1 / ERK2 Polyclonal AntibodyA16686WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
ImmunogenA synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
Recombinant Protein of human MAPK1/MAPK3
SequenceFLTEYVA
Gene ID5594/5595
Swiss protP28482/P27361
Synonyms
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42kDa/44kDa
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular location
Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)
ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:5000 dilution or ERK1/2 antibody (A16686).293T cells and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded rat brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded rat heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded human tonsil using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded human uterine cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunohistochemistry of paraffin-embedded mouse heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (AP0472) at dilution of 1:100 (40x lens).

ABclonal:Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) }
Immunoprecipitation - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472). Western blot was performed from the immunoprecipitate using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.


ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)

Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (A16686) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunohistochemistry of paraffin-embedded human lung cancer using ERK1 / ERK2 antibody (A16686) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunohistochemistry of paraffin-embedded human uterine cancer using ERK1 / ERK2 antibody (A16686) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunohistochemistry of paraffin-embedded mouse kidney using ERK1 / ERK2 antibody (A16686) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunofluorescence analysis of C6 cells using ERK1 / ERK2 antibody (A16686) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 antibody (A16686) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunofluorescence analysis of U2OS cells using ERK1 / ERK2 antibody (A16686) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunofluorescence analysis of C6 cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunofluorescence analysis of NIH-3T3 cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Immunofluorescence analysis of U-2 OS cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }
Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Confocal immunofluorescence analysis of Hela cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:200. Blue: DAPI for nuclear staining.

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