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Phospho-CREB1-S133 Antibody Kit (RK04087)

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ABclonal:Western blot - Phospho-CREB1-S133 pAb (AP0019)
ABclonal:Immunofluorescence - Phospho-CREB1-S133 pAb (AP0019)
ABclonal:Immunoprecipitation - Phospho-CREB1-S133 pAb (AP0019)
ABclonal:Western blot - CREB1 Polyclonal Antibody (A11989)
ABclonal:Immunofluorescence - CREB1 Polyclonal Antibody (A11989)

Overview

Product namePhospho-CREB1-S133 Antibody Kit
Catalog No.RK04087

Product Component

Product nameCatalog No.Positive ApplicationsSpecies Reactivity
Phospho-CREB1-S133 pAbAP0019WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Rat
CREB1 Polyclonal AntibodyA11989WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayHuman, Mouse, Rat
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
ImmunogenA phospho specific peptide corresponding to residues surrounding Ser133 of human CREB
Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human CREB1 (NP_604391.1).
SequenceEmail for sequence
Gene ID1385
Swiss protP16220
SynonymsCREB; CREB-1
Calculated MW25kDa/35kDa/36kDa
Observed MW43kDa
Storage bufferStore at -20℃. Avoid freeze/ thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationNucleus
Phospho-CREB1-S133 pAb (AP0019)
ABclonal:Western blot - Phospho-CREB1-S133 pAb (AP0019) }
Western blot - Phospho-CREB1-S133 pAb (AP0019)

Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 antibody (AP0019) at 1:1000 dilution or CREB1 antibody (A11989). HeLa cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Phospho-CREB1-S133 pAb (AP0019) }
Immunofluorescence - Phospho-CREB1-S133 pAb (AP0019)

Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 antibody (AP0019). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Phospho-CREB1-S133 pAb (AP0019) }
Immunoprecipitation - Phospho-CREB1-S133 pAb (AP0019)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-CREB1-S133 pAb (AP0019). Western blot was performed from the immunoprecipitate using Phospho-CREB1-S133 pAb (AP0019) at a dilition of 1:1000. 293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.


CREB1 Polyclonal Antibody (A11989)
ABclonal:Western blot - CREB1 Polyclonal Antibody (A11989) }
Western blot - CREB1 Polyclonal Antibody (A11989)

Western blot analysis of extracts of various cell lines, using CREB1 antibody (A11989) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - CREB1 Polyclonal Antibody (A11989) }
Immunofluorescence - CREB1 Polyclonal Antibody (A11989)

Immunofluorescence analysis of HeLa cells using CREB1 antibody (A11989) at dilution of 1:100. Blue: DAPI for nuclear staining.

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