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p70 S6 Kinase 1 Rabbit pAb (A2190)

Reviews (5)Publications (7) Datasheet

ABclonal:Western blot - p70 S6 Kinase 1 Rabbit pAb (A2190)

Western blot analysis of extracts of various cell lines, using p70 S6 Kinase 1 1 antibody (A2190) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunohistochemistry of paraffin-embedded human breast cancer using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunofluorescence analysis of C6 cells using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunofluorescence analysis of NIH-3T3 cells using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunofluorescence analysis of U-2 OS cells using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for p70 S6 Kinase 1 Rabbit pAb(A2190)
Review
ABclonal: review for p70 S6 Kinase 1 Rabbit pAb(A2190)
Review
ABclonal: review for p70 S6 Kinase 1 Rabbit pAb(A2190)
Review
ABclonal: review for p70 S6 Kinase 1 Rabbit pAb(A2190)
Review
ABclonal: review for p70 S6 Kinase 1 Rabbit pAb(A2190)

Overview

Product namep70 S6 Kinase 1 Rabbit pAb
Catalog No.A2190
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human P70 S6K (NP_003152.1).
SequenceMRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFF
Gene ID6198
Swiss protP23443
SynonymsPS6K; S6K; S6K-beta-1; S6K1; STK14A; p70 S6KA; p70(S6K)-alpha; p70-S6K; p70-alpha; P70 S6K; RPS6KB1; p70S6KA
Calculated MW51kDa/52kDa/56kDa/59kDa
Observed MW70KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, 293T
Cellular locationCell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome
Customer validation

(Human bone marrow cancer cell, Human chronic myeloid leukemia cells, HeLa cells, HEK293T)

WB(Homo sapiens, Rattus norvegicus, Spodoptera frugiperda, Macaca mulatta, Mus musculus)

ABclonal:Western blot - p70 S6 Kinase 1 Rabbit pAb (A2190) }

Western blot - p70 S6 Kinase 1 Rabbit pAb (A2190)

Western blot analysis of extracts of various cell lines, using p70 S6 Kinase 1 1 antibody (A2190) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - p70 S6 Kinase 1 Rabbit pAb (A2190) }

Immunohistochemistry - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunohistochemistry of paraffin-embedded human breast cancer using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190) }

Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunofluorescence analysis of C6 cells using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190) }

Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunofluorescence analysis of NIH-3T3 cells using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190) }

Immunofluorescence - p70 S6 Kinase 1 Rabbit pAb (A2190)

Immunofluorescence analysis of U-2 OS cells using p70 S6 Kinase 1 1 Rabbit pAb (A2190) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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