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p53 Rabbit pAb (A5761)

Reviews (3)Publications (18) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Immunoprecipitation - p53 Rabbit pAb (A5761)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug p53 antibody (A5761). Western blot was performed from the immunoprecipitate using p53 antibody (A5761) at a dilition of 1:500.

Review
ABclonal: review for p53 Rabbit pAb(A5761)
Review
ABclonal: review for p53 Rabbit pAb(A5761)
Review
ABclonal: review for p53 Rabbit pAb(A5761)

Overview

Product namep53 Rabbit pAb
Catalog No.A5761
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human p53 (NP_000537.3).
SequenceMEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQ
Gene ID7157
Swiss protP04637
SynonymsBCC7; LFS1; P53; TRP53; p53; TP53; BMFS5
Calculated MW43kDa
Observed MW53KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:3000 - 1:6000
  • IHC 1:50 - 1:200
  • IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesNIH/3T3, Rat lung
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

(Hepatoma cell line, Rat cells, MEF)

WB(Homo sapiens, Rattus norvegicus, Lampetra japonica, Mus musculus, Sus scrofa)

IF(Lampetra japonica)

IHC(Homo sapiens)

ABclonal:Immunoprecipitation - p53 Rabbit pAb (A5761)}

Immunoprecipitation - p53 Rabbit pAb (A5761)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug p53 antibody (A5761). Western blot was performed from the immunoprecipitate using p53 antibody (A5761) at a dilition of 1:500.

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