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p53 Rabbit pAb (A0263)

Reviews (2)Publications (21) Datasheet

ABclonal:Western blot - p53 Rabbit pAb (A0263)

Western blot analysis of extracts of 293T cells, using p53 Rabbit pAb (A0263) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A0263) at dilution of 1:150 (40x lens).

ABclonal:Immunofluorescence - p53 Rabbit pAb (A0263)

Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - p53 Rabbit pAb (A0263)

Confocal immunofluorescence analysis of U-2 OS cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - p53 Rabbit pAb (A0263)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug HK1 antibody (A0263). Western blot was performed from the immunoprecipitate using HK1 (A0263) at a dilition of 1:3000.

ABclonal:Chromatin Immunoprecipitation - p53 Rabbit pAb (A0263)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (A0263) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for p53 Rabbit pAb(A0263)
Review
ABclonal: review for p53 Rabbit pAb(A0263)

Overview

Product namep53 Rabbit pAb
Catalog No.A0263
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human p53 (NP_000537.3).
SequenceMEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFR
Gene ID7157
Swiss protP04637
SynonymsBCC7; LFS1; P53; TRP53; p53; TP53; BMFS5
Calculated MW23-43kDa
Observed MW53KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:50 - 1:100
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

(Human cells, SW480)

WB(Homo sapiens, Mus musculus, Gallus gallus, Danio rerio, Rattus norvegicus)

IHC(Mus musculus)

IHC(Rattus norvegicus)

ABclonal:Western blot - p53 Rabbit pAb (A0263) }

Western blot - p53 Rabbit pAb (A0263)

Western blot analysis of extracts of 293T cells, using p53 Rabbit pAb (A0263) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263) }

Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A0263) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - p53 Rabbit pAb (A0263) }

Immunofluorescence - p53 Rabbit pAb (A0263)

Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - p53 Rabbit pAb (A0263) }

Immunofluorescence - p53 Rabbit pAb (A0263)

Confocal immunofluorescence analysis of U-2 OS cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - p53 Rabbit pAb (A0263) }

Immunoprecipitation - p53 Rabbit pAb (A0263)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug HK1 antibody (A0263). Western blot was performed from the immunoprecipitate using HK1 (A0263) at a dilition of 1:3000.
ABclonal:Chromatin Immunoprecipitation - p53 Rabbit pAb (A0263) }

Chromatin Immunoprecipitation - p53 Rabbit pAb (A0263)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (A0263) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

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