Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

p53 Rabbit pAb (A0263)

Reviews (2)Publications (45) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Rat

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry of paraffin-embedded Human colon (negative control sample) using p53 Rabbit pAb (A0263) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A0263) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - p53 Rabbit pAb (A0263)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug HK1 antibody (A0263). Western blot was performed from the immunoprecipitate using HK1 (A0263) at a dilution of 1:3000.

Review
ABclonal: review for p53 Rabbit pAb(A0263)
Review
ABclonal: review for p53 Rabbit pAb(A0263)

Overview

Product namep53 Rabbit pAb
Catalog No.A0263
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (P04637).
SequenceMEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
Gene ID7157
Swiss protP04637
SynonymsBCC7; LFS1; P53; TRP53; p53; TP53; BMFS5
Calculated MW23-43kDa
Observed MW53KDa

Applications

ReactivityHuman, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

(Human cells, SW480)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Danio rerio, Megalobrama amblycephala, Sus scrofa)

IHC(Rattus norvegicus)

IHC(Mus musculus)

Co-IP(Homo sapiens)

ICC(Homo sapiens)

IF(Rattus norvegicus)

IF(Homo sapiens)

Research Area

p53 Rabbit pAb images

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263)}

Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry of paraffin-embedded Human colon (negative control sample) using p53 Rabbit pAb (A0263) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A0263)}

Immunohistochemistry - p53 Rabbit pAb (A0263)

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 Rabbit pAb (A0263) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - p53 Rabbit pAb (A0263)}

Immunoprecipitation - p53 Rabbit pAb (A0263)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug HK1 antibody (A0263). Western blot was performed from the immunoprecipitate using HK1 (A0263) at a dilution of 1:3000.

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Related Targets

Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53

* Data provided by citexs.com, for reference only.

Publishing research using A0263? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (29)

ELISA Kits (1)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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