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p53 Rabbit pAb (A5761)

Reviews (3)Publications (11) Datasheet

ABclonal:Western blot - p53 Rabbit pAb (A5761)
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A5761)
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A5761)
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A5761)
ABclonal:Immunoprecipitation - p53 Rabbit pAb (A5761)
ABclonal:Chromatin Immunoprecipitation - p53 Rabbit pAb (A5761)
Review
ABclonal: review for p53 Rabbit pAb(A5761)
Review
ABclonal: review for p53 Rabbit pAb(A5761)
Review
ABclonal: review for p53 Rabbit pAb(A5761)

Overview

Product namep53 Rabbit pAb
Catalog No.A5761
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human p53 (NP_000537.3).
SequenceEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD
Gene ID22059
Swiss protP02340
SynonymsTP53; BCC7; LFS1; P53; TRP53; Trp53; bbl; bfy; bhy; p44; p53; Tp53
Calculated MW43kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:3000 - 1:6000
IHC 1:50 - 1:200
IP 1:20 - 1:50
ChIP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesA-549, BT-474, MCF7, Mouse spleen, Mouse testis, Mouse kidney, Rat kidney
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

(MEF, Hepatoma cell line, Rat cells)

WB(Homo sapiens,Mus musculus, Rattus norvegicus, Lampetra japonica)

IF(Lampetra japonica)

    ABclonal:Western blot - p53 Rabbit pAb (A5761) }

    Western blot - p53 Rabbit pAb (A5761)

    Western blot analysis of extracts of various cell lines, using p53 antibody (A5761) at 1:6000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.
    ABclonal:Immunohistochemistry - p53 Rabbit pAb (A5761) }

    Immunohistochemistry - p53 Rabbit pAb (A5761)

    Immunohistochemistry of paraffin-embedded rat ovary using p53 antibody (A5761) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - p53 Rabbit pAb (A5761) }

    Immunohistochemistry - p53 Rabbit pAb (A5761)

    Immunohistochemistry of paraffin-embedded human lung cancer using p53 antibody (A5761) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - p53 Rabbit pAb (A5761) }

    Immunohistochemistry - p53 Rabbit pAb (A5761)

    Immunohistochemistry of paraffin-embedded human gastric cancer using p53 antibody (A5761) at dilution of 1:100 (40x lens).
    ABclonal:Immunoprecipitation - p53 Rabbit pAb (A5761) }

    Immunoprecipitation - p53 Rabbit pAb (A5761)

    Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug p53 antibody (A5761). Western blot was performed from the immunoprecipitate using p53 antibody (A5761) at a dilition of 1:500.
    ABclonal:Chromatin Immunoprecipitation - p53 Rabbit pAb (A5761) }

    Chromatin Immunoprecipitation - p53 Rabbit pAb (A5761)

    Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (A5761) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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