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p53 Polyclonal Antibody (A0263)

Reviews (2)Publications (4) Datasheet

ABclonal:Western blot - p53 Polyclonal Antibody (A0263)
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A0263)
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A0263)
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A0263)
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A0263)
ABclonal:Chromatin Immunoprecipitation - p53 Polyclonal Antibody (A0263)
Review
ABclonal: review for p53 Polyclonal Antibody(A0263)
Review
ABclonal: review for p53 Polyclonal Antibody(A0263)

Overview

Product namep53 Polyclonal Antibody
Catalog No.A0263
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human p53 (NP_000537.3).
SequenceMEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFR
Gene ID7157
Swiss protP04637
SynonymsTP53; BCC7; LFS1; P53; TRP53; tumor protein p53
Calculated MW23-43kDa
Observed MW53kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, U-251MG
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

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WB(Human, Human, Mouse)

ABclonal:Western blot - p53 Polyclonal Antibody (A0263) }

Western blot - p53 Polyclonal Antibody (A0263)

Western blot analysis of extracts of various cell lines, using p53 antibody (A0263) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - p53 Polyclonal Antibody (A0263) }

Immunohistochemistry - p53 Polyclonal Antibody (A0263)

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 antibody (A0263) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A0263) }

Immunofluorescence - p53 Polyclonal Antibody (A0263)

Immunofluorescence analysis of A549 cells using p53 antibody (A0263).
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A0263) }

Immunofluorescence - p53 Polyclonal Antibody (A0263)

Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - p53 Polyclonal Antibody (A0263) }

Immunofluorescence - p53 Polyclonal Antibody (A0263)

Confocal immunofluorescence analysis of U-2 OS cells using p53 Polyclonal Antibody (A0263) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - p53 Polyclonal Antibody (A0263) }

Chromatin Immunoprecipitation - p53 Polyclonal Antibody (A0263)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (A0263) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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