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p38 MAPK Polyclonal Antibody (A0227)

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ABclonal:Western blot - p38 MAPK Polyclonal Antibody (A0227)
Review
ABclonal: review for p38 MAPK Polyclonal Antibody(A0227)
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ABclonal: review for p38 MAPK Polyclonal Antibody(A0227)
Review
ABclonal: review for p38 MAPK Polyclonal Antibody(A0227)

Overview

Product namep38 MAPK Polyclonal Antibody
Catalog No.A0227
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human p38 MAPK (NP_620581.1).
SequenceMSQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES
Gene ID1432
Swiss protQ16539
SynonymsMAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; mitogen-activated protein kinase 14
Calculated MW29kDa/34kDa/35kDa/41kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSW620, HL-60, HepG2, MCF7, 293T, Mouse liver, Mouse skeletal muscle, Mouse spleen, Mouse kidney
Cellular locationCytoplasm, Nucleus
Customer validation

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WB(Human, Mouse, Rat)

IF(Mouse)

ABclonal:Western blot - p38 MAPK Polyclonal Antibody (A0227) }

Western blot - p38 MAPK Polyclonal Antibody (A0227)

Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (A0227) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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