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[One Step]MAPK1 Antibody Kit (RK05664)




Product name[One Step]MAPK1 Antibody Kit
Catalog No.RK05664

Product Component

ComponentNameRecommended DilutionApplicationsSpecies Reactivity
Primary Antibody[KO Validated] ERK2 Rabbit pAb1:1000 dilutionWBHuman, Mouse, Rat
Secondary AntibodyHRP Goat Anti-Rabbit IgG (H+L)1:4000 dilution
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_620407.1).
Gene ID5594
Swiss protP28482
SynonymsERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; MAPK1
Calculated MW36kDa/41kDa
Observed MW42KDa
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular locationCytoplasm,Nucleus,centrosome,cytoskeleton,microtubule organizing center,spindle

Western blot analysis of extracts of various cells, using MAPK1 antibody (A11186) at 1:1000 dilution ratio through one-step method.

* For research use only. Not for therapeutic or diagnostic purposes.

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