Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

mTOR Rabbit pAb (A2445)

Review (1)Publications (59) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - mTOR Rabbit pAb (A2445)

Western blot analysis of extracts of K-562 cells, using mTOR antibody (A2445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Western blot - mTOR Rabbit pAb (A2445)

Western blot analysis of various lysates, using mTOR antibody (A2445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of HeLa cells using mTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of PC12 cells using mTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of C6 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - mTOR Rabbit pAb (A2445)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody (A2445). Western blot was performed from the immunoprecipitate using mTOR antibody (A2445) at a dilution of 1:1000.

Review
ABclonal: review for mTOR Rabbit pAb(A2445)

Overview

Product namemTOR Rabbit pAb
Catalog No.A2445
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR (NP_004949.1).
SequenceMLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
Gene ID2475
Swiss protP42345
SynonymsFRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mTOR; MTOR
Calculated MW288kDa
Observed MW289KDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesK-562, C6
Cellular locationCytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein
Customer validation

(unknow)

WB(Sus scrofa, Rattus norvegicus, Homo sapiens, Mus musculus, Spodoptera frugiperda, Capra hircus, Gallus gallus, Ovis aries)

Research Area

mTOR Rabbit pAb images

ABclonal:Western blot - mTOR Rabbit pAb (A2445)}

Western blot - mTOR Rabbit pAb (A2445)

Western blot analysis of extracts of K-562 cells, using mTOR antibody (A2445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Western blot - mTOR Rabbit pAb (A2445)}

Western blot - mTOR Rabbit pAb (A2445)

Western blot analysis of various lysates, using mTOR antibody (A2445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)}

Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of HeLa cells using mTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)}

Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of PC12 cells using mTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)}

Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of C6 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - mTOR Rabbit pAb (A2445)}

Immunofluorescence - mTOR Rabbit pAb (A2445)

Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - mTOR Rabbit pAb (A2445)}

Immunoprecipitation - mTOR Rabbit pAb (A2445)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody (A2445). Western blot was performed from the immunoprecipitate using mTOR antibody (A2445) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on mTOR . (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to mTOR . (Distance between topics and target gene indicate popularity.) mTOR

* Data provided by citexs.com, for reference only.

Publishing research using A2445? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (5)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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