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mTOR Rabbit pAb (A11354)

Review (1)Publications (2) Datasheet

ABclonal:Western blot - mTOR Rabbit pAb (A11354)

Western blot analysis of extracts of various cell lines, using mTOR antibody (A11354) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - mTOR Rabbit pAb (A11354)

Immunohistochemistry of paraffin-embedded rat ovary using mTOR antibody (A11354) at dilution of 1:150 (40x lens).

ABclonal:Immunohistochemistry - mTOR Rabbit pAb (A11354)

Immunohistochemistry of paraffin-embedded human placenta using mTOR antibody (A11354) at dilution of 1:150 (40x lens).

ABclonal:Immunohistochemistry - mTOR Rabbit pAb (A11354)

Immunohistochemistry of paraffin-embedded mouse kidney using mTOR antibody (A11354) at dilution of 1:150 (40x lens).

ABclonal:Immunofluorescence - mTOR Rabbit pAb (A11354)

Immunofluorescence analysis of C6 cells using mTOR Rabbit pAb (A11354) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - mTOR Rabbit pAb (A11354)

Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (A11354) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for mTOR Rabbit pAb(A11354)

Overview

Product namemTOR Rabbit pAb
Catalog No.A11354
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2388-2487 of human mTOR (NP_004949.1).
SequenceEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGL
Gene ID2475
Swiss protP42345
SynonymsFRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mTOR; MTOR
Calculated MW288kDa
Observed MW300kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, U-87MG
Cellular locationCytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein
Customer validation

(MSC cells)

WB(Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - mTOR Rabbit pAb (A11354) }

Western blot - mTOR Rabbit pAb (A11354)

Western blot analysis of extracts of various cell lines, using mTOR antibody (A11354) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - mTOR Rabbit pAb (A11354) }

Immunohistochemistry - mTOR Rabbit pAb (A11354)

Immunohistochemistry of paraffin-embedded rat ovary using mTOR antibody (A11354) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - mTOR Rabbit pAb (A11354) }

Immunohistochemistry - mTOR Rabbit pAb (A11354)

Immunohistochemistry of paraffin-embedded human placenta using mTOR antibody (A11354) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - mTOR Rabbit pAb (A11354) }

Immunohistochemistry - mTOR Rabbit pAb (A11354)

Immunohistochemistry of paraffin-embedded mouse kidney using mTOR antibody (A11354) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - mTOR Rabbit pAb (A11354) }

Immunofluorescence - mTOR Rabbit pAb (A11354)

Immunofluorescence analysis of C6 cells using mTOR Rabbit pAb (A11354) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - mTOR Rabbit pAb (A11354) }

Immunofluorescence - mTOR Rabbit pAb (A11354)

Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (A11354) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

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