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mTOR Polyclonal Antibody (A2445)

Review (1)Publications (7) Datasheet

ABclonal:Western blot - mTOR Polyclonal Antibody (A2445)
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445)
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445)
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445)
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445)
ABclonal:Immunoprecipitation - mTOR Polyclonal Antibody (A2445)
Review
ABclonal: review for mTOR Polyclonal Antibody(A2445)

Overview

Product namemTOR Polyclonal Antibody
Catalog No.A2445
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR (NP_004949.1).
SequenceMLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
Gene ID2475
Swiss protP42345
SynonymsMTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mechanistic target of rapamycin
Calculated MW288kDa
Observed MW289kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, K-562, U-87MG
Cellular locationCytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein
Customer validation

WB(Pig, mouse, Rat, human, Pigs)

ABclonal:Western blot - mTOR Polyclonal Antibody (A2445) }

Western blot - mTOR Polyclonal Antibody (A2445)

Western blot analysis of extracts of various cell lines, using mTOR antibody (A2445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445) }

Immunofluorescence - mTOR Polyclonal Antibody (A2445)

Immunofluorescence analysis of HeLa cells using mTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445) }

Immunofluorescence - mTOR Polyclonal Antibody (A2445)

Immunofluorescence analysis of PC12 cells using mTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445) }

Immunofluorescence - mTOR Polyclonal Antibody (A2445)

Immunofluorescence analysis of C6 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - mTOR Polyclonal Antibody (A2445) }

Immunofluorescence - mTOR Polyclonal Antibody (A2445)

Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (A2445) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - mTOR Polyclonal Antibody (A2445) }

Immunoprecipitation - mTOR Polyclonal Antibody (A2445)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody (A2445). Western blot was performed from the immunoprecipitate using mTOR antibody (A2445) at a dilition of 1:1000.

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