Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] c-Myc Rabbit pAb (A1309)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using c-Myc antibody (A1309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of extracts from normal (control) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody (A1309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review

Overview

Product name	[KO Validated] c-Myc Rabbit pAb
Catalog No.	A1309
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 347-439 of human c-Myc (NP_002458.2).
Sequence	VLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRR
Gene ID	4609
Swiss prot	P01106
Synonyms	MRTL; MYCC; bHLHe39; c-Myc; MYC
Calculated MW	48kDa/50kDa
Observed MW	60KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting
Positive samples	HCT116, MCF7, NIH/3T3, Mouse liver
Cellular location	Nucleus, nucleolus, nucleoplasm
Customer validation	(unknow, Human Bladder Cancer) WB(Homo sapiens, Gallus gallus, Mus musculus, Sus scrofa) Co-IP(Homo sapiens,Gallus gallus, Homo sapiens, Mus musculus) ChIP(Homo sapiens,Mus musculus) IP(Homo sapiens,Mus musculus)

Research Area

[KO Validated] c-Myc Rabbit pAb images

Western blot - [KO Validated] c-Myc Rabbit pAb (A1309)

Submit an review for A1309

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Reviews List

Product Protocols

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References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

tumor biology

Application:

IF:

3.355

Species:

Homo sapiens

PMID:

28671049

Title:

Clinicopathological significance of c-MYC in esophageal squamous cell carcinoma

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

Application:

WB, Co-IP

IF:

5.293

Species:

Homo sapiens,Gallus gallus

PMID:

29670864

Title:

Mechanism of Apoptosis Induction by Mycoplasmal Nuclease MGA_0676 in Chicken Embryo Fibroblasts

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Hepatology (Baltimore, Md.)

Application:

ChIP, Co-IP

IF:

17.425

Species:

Homo sapiens,Mus musculus

PMID:

30180281

Title:

Hepatitis B Virus Induces Autophagy to Promote its Replication by the Axis of miR‐192‐3p‐XIAP via NF‐κB Signaling

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

30407707

Title:

MiR-520b promotes the progression of non-small cell lung cancer through activating Hedgehog pathway.

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Oncogenesis

Application:

IF:

7.485

Species:

Mus musculus

PMID:

30573731

Title:

CD36 plays a critical role in proliferation, migration and tamoxifen-inhibited growth of ER-positive breast cancer cells.

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

6.543

Species:

Mus musculus

PMID:

31531187

Title:

Betulinic Acid Suppresses Breast Cancer Metastasis by Targeting GRP78-Mediated Glycolysis and ER Stress Apoptotic Pathway.

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Nature communications

Application:

IF:

14.919

Species:

Homo sapiens,Mus musculus

PMID:

31484922

Title:

CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Nature communications

Application:

IF:

14.919

Species:

Mus musculus

PMID:

31484922

Title:

CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

American Journal of Physiology. Gastrointestinal and Liver Physiology

Application:

IF:

4.052

Species:

Homo sapiens

PMID:

31604033

Title:

Hepatitis B virus-triggered PTEN/β-catenin/c-Myc signaling enhances PD-L1 expression to promote immune evasion

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

International Journal of Molecular Sciences

Application:

IF:

5.923

Species:

Mus musculus

PMID:

28749413

Title:

PGK1 Drives Hepatocellular Carcinoma Metastasis by Enhancing Metabolic Process.

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Cell Cycle

Application:

IF:

4.534

Species:

Sus scrofa

PMID:

29119857

Title:

miR-638 Inhibits immature Sertoli cell growth by indirectly inactivating PI3K/AKT pathway via SPAG1 gene.

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Protein Cell

Application:

IF:

14.87

Species:

Homo sapiens

PMID:

32748349

Title:

KIF2C: a novel link between Wnt/尾-catenin and mTORC1 signaling in the pathogenesis of hepatocellular carcinoma

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Reprod Domest Anim

Application:

IF:

2.005

Species:

Sus scrofa

PMID:

33305371

Title:

AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR‐124a

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Adv Sci (Weinh)

Application:

IF:

16.8

Species:

Mus musculus

PMID:

34026434

Title:

PRMT5 Enables Robust STAT3 Activation via Arginine Symmetric Dimethylation of SMAD7

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Aging (Albany NY)

Application:

IF:

5.682

Species:

Homo sapiens

PMID:

33099540

Title:

CUEDC1 inhibits epithelial-mesenchymal transition via the TβRI/Smad signaling pathway and suppresses tumor progression in non-small cell lung cancer

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Neoplasia

Application:

IF:

5.715

Species:

Homo sapiens

PMID:

34325342

Title:

PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

3 Biotech

Application:

IF:

2.406

Species:

Homo sapiens

PMID:

34458056

Title:

Tumor-promoting function of PIMREG in glioma by activating the β-catenin pathway

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

Cell Rep

Application:

WB, IP

IF:

9.423

Species:

Homo sapiens,Mus musculus

PMID:

34758320

Title:

Phosphorylation at Ser68 facilitates DCAF11-mediated ubiquitination and degradation of CENP-A during the cell cycle

References for [KO Validated] c-Myc Rabbit pAb (A1309)

Product:

[KO Validated] c-Myc Rabbit pAb

Journal:

J Agric Food Chem

Application:

IF:

5.279

Species:

Mus musculus

PMID:

34928143

Title:

Characterizations and the Mechanism Underlying Osteogenic Activity of Peptides from Enzymatic Hydrolysates of Stichopus japonicus

Publishing research using A1309? Please let us know so that we can cite the reference in this datasheet.

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Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] c-Myc Rabbit pAb (A1309)

Overview

Background

Immunogen information

Applications

Research Area

[KO Validated] c-Myc Rabbit pAb images

Submit an review for A1309

Reviews List

Product Protocols

Inquire About This Product

Catalog Antibodies (8)

Secondary Antibodies (10)

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