Western blot analysis of extracts of various cell lines, using cIAP2 antibody (A0833) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens).
Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
| Product name | cIAP2 Rabbit pAb |
|---|---|
| Catalog No. | A0833 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human cIAP2 (NP_001156.1). |
|---|---|
| Sequence | MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWP |
| Gene ID | 330 |
| Swiss prot | Q13489 |
| Synonyms | BIRC3; AIP1; API2; CIAP2; HAIP1; HIAP1; MALT2; MIHC; RNF49; c-IAP2 |
| Calculated MW | 68kDa |
| Observed MW | 75KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HepG2, RAW264.7 |
| Cellular location | Cytoplasm, Nucleus |
| Customer validation | WB(Homo sapiens) |
* For research use only. Not for therapeutic or diagnostic purposes.
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