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cIAP2 Rabbit pAb (A0833)

Publications (2) Datasheet

ABclonal:Western blot - cIAP2 Rabbit pAb (A0833)

Western blot analysis of extracts of various cell lines, using cIAP2 antibody (A0833) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - cIAP2 Rabbit pAb (A0833)

Immunohistochemistry of paraffin-embedded human colon carcinoma using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens).

ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of NIH-3T3 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namecIAP2 Rabbit pAb
Catalog No.A0833
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human cIAP2 (NP_001156.1).
SequenceMNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWP
Gene ID330
Swiss protQ13489
SynonymsBIRC3; AIP1; API2; CIAP2; HAIP1; HIAP1; MALT2; MIHC; RNF49; c-IAP2
Calculated MW68kDa
Observed MW75KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, RAW264.7
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - cIAP2 Rabbit pAb (A0833) }

Western blot - cIAP2 Rabbit pAb (A0833)

Western blot analysis of extracts of various cell lines, using cIAP2 antibody (A0833) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - cIAP2 Rabbit pAb (A0833) }

Immunohistochemistry - cIAP2 Rabbit pAb (A0833)

Immunohistochemistry of paraffin-embedded human colon carcinoma using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833) }

Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833) }

Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of NIH-3T3 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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