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cIAP1 Rabbit pAb (A0866)

Datasheet

ABclonal:Western blot - cIAP1 Rabbit pAb (A0866)
ABclonal:Immunohistochemistry - cIAP1 Rabbit pAb (A0866)
ABclonal:Immunofluorescence - cIAP1 Rabbit pAb (A0866)

Overview

Product namecIAP1 Rabbit pAb
Catalog No.A0866
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human cIAP1 (NP_001157.1).
SequenceMHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGR
Gene ID329
Swiss protQ13490
SynonymsAPI1; HIAP2; Hiap-2; MIHB; RNF48; c-IAP1; cIAP1; BIRC2
Calculated MW64kDa/69kDa
Observed MW70KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, Rat testis
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - cIAP1 Rabbit pAb (A0866) }

Western blot - cIAP1 Rabbit pAb (A0866)

Western blot analysis of extracts of various cell lines, using cIAP1 antibody (A0866) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - cIAP1 Rabbit pAb (A0866) }

Immunohistochemistry - cIAP1 Rabbit pAb (A0866)

Immunohistochemistry of paraffin-embedded rat testis using cIAP1 Rabbit pAb (A0866) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - cIAP1 Rabbit pAb (A0866) }

Immunofluorescence - cIAP1 Rabbit pAb (A0866)

Immunofluorescence analysis of A549 cells using cIAP1 antibody (A0866).

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