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cIAP1 Rabbit mAb (A19688)

Datasheet

ABclonal:Western blot - cIAP1 Rabbit mAb (A19688)
ABclonal:Immunohistochemistry - cIAP1 Rabbit mAb (A19688)

Overview

Product namecIAP1 Rabbit mAb
Catalog No.A19688
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
ImmunogenA synthesized peptide derived from human cIAP1.
SequenceEmail for sequence
Gene ID329
Swiss protQ13490
SynonymsAPI1; HIAP2; Hiap-2; MIHB; RNF48; c-IAP1; cIAP1; BIRC2
Calculated MW70kDa
Observed MW68KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, BxPC-3, HeLa, Jurkat
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - cIAP1 Rabbit mAb (A19688) }

Western blot - cIAP1 Rabbit mAb (A19688)

Western blot analysis of extracts of various cell lines, using cIAP1 antibody (A19688) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - cIAP1 Rabbit mAb (A19688) }

Immunohistochemistry - cIAP1 Rabbit mAb (A19688)

Immunohistochemistry of paraffin-embedded rat ovary using cIAP1 antibody (A19688) at dilution of 1:100 (40x lens).

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