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cFLIP Rabbit pAb (A2555)

Review (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - cFLIP Rabbit pAb (A2555)

Western blot analysis of extracts of Jurkat cells, using cFLIP antibody (A2555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - cFLIP Rabbit pAb (A2555)

Western blot analysis of extracts of various cell lines, using cFLIP antibody (A2555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for cFLIP Rabbit pAb(A2555)

Overview

Product namecFLIP Rabbit pAb
Catalog No.A2555
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 90-240 of human cFLIP (NP_003870.4).
SequenceVSDYRVLMAEIGEDLDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQSVQGAGTSYRNVLQAAIQKSLKDPSNNFRLHNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIP
Gene ID8837
Swiss protO15519
SynonymsCFLAR; CASH; CASP8AP1; CLARP; Casper; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS
Calculated MW23-55kDa
Observed MW28kDa/55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesJurkat, Mouse liver, Mouse heart, Rat liver, Rat heart
Cellular location
Customer validation

(Human fibrosarcoma cells)

ABclonal:Western blot - cFLIP Rabbit pAb (A2555) }

Western blot - cFLIP Rabbit pAb (A2555)

Western blot analysis of extracts of Jurkat cells, using cFLIP antibody (A2555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - cFLIP Rabbit pAb (A2555) }

Western blot - cFLIP Rabbit pAb (A2555)

Western blot analysis of extracts of various cell lines, using cFLIP antibody (A2555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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