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alpha smooth muscle actin Rabbit pAb (A7248)

Publications (4) Datasheet

ABclonal:Western blot - alpha smooth muscle actin Rabbit pAb (A7248)
ABclonal:Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248)
ABclonal:Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248)
ABclonal:Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248)

Overview

Product namealpha smooth muscle actin Rabbit pAb
Catalog No.A7248
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human alpha smooth muscle actin (NP_001135417.1).
SequenceMCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF
Gene ID59
Swiss protP62736
SynonymsAAT6; ACTSA; MYMY5; ACTA2; actin
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesSW480, Mouse spleen, Rat spleen
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - alpha smooth muscle actin Rabbit pAb (A7248) }

Western blot - alpha smooth muscle actin Rabbit pAb (A7248)

Western blot analysis of extracts of various cell lines, using alpha smooth muscle actin antibody (A7248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248) }

Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248)

Immunofluorescence analysis of rat lung using alpha smooth muscle actin antibody (A7248). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248) }

Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248)

Confocal immunofluorescence analysis of human colon carcinoma using alpha smooth muscle actin Polyclonal Antibody (A7248) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248) }

Immunofluorescence - alpha smooth muscle actin Rabbit pAb (A7248)

Confocal immunofluorescence analysis of mouse colon using alpha smooth muscle actin Polyclonal Antibody (A7248) at dilution of 1:100. Blue: DAPI for nuclear staining.

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