Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat
Western blot analysis of extracts of various cell lines, using Alpha-tubulin (ubiquitous) chain antibody (AC024) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | Alpha-tubulin (ubiquitous) chain Rabbit pAb |
|---|---|
| Catalog No. | AC024 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence within amino acids 1-80 of human Alpha-tubulin (ubiquitous) chain (NP_006073.2). |
|---|---|
| Sequence | MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRT |
| Gene ID | 10376 |
| Swiss prot | P68363 |
| Synonyms | TUBA1B; K-ALPHA-1 |
| Calculated MW | 37kDa/50kDa |
| Observed MW | 52kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | HeLa, PC-12, A-549, U-251MG, Mouse brain |
| Cellular location | Cytoplasm, cytoskeleton |
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Based on existing publications, the following genes are closely related to research on TUBA1B. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to TUBA1B. (Distance between topics and target gene indicate popularity.) TUBA1B
* Data provided by citexs.com, for reference only.
Publishing research using AC024? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
