Product Type > Antibodies > Tag and Loading Control Antibodies

Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Western blot analysis of extracts of various cell lines, using Alpha-tubulin (ubiquitous) chain antibody (AC024) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunohistochemistry of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunohistochemistry of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunofluorescence analysis of U2OS cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameAlpha-tubulin (ubiquitous) chain Rabbit pAb
Catalog No.AC024
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence within amino acids 1-80 of human Alpha-tubulin (ubiquitous) chain (NP_006073.2).
SequenceMRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRT
Gene ID10376
Swiss protP68363
SynonymsTUBA1B; K-ALPHA-1
Calculated MW37kDa/50kDa
Observed MW52kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:100 - 1:500
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, PC-12, A-549, U-251MG, Mouse brain
Cellular locationCytoplasm, cytoskeleton

Research Area

Alpha-tubulin (ubiquitous) chain Rabbit pAb images

ABclonal:Western blot - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)}

Western blot - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Western blot analysis of extracts of various cell lines, using Alpha-tubulin (ubiquitous) chain antibody (AC024) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)}

Immunohistochemistry - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunohistochemistry of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)}

Immunohistochemistry - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunohistochemistry of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)}

Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)}

Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)}

Immunofluorescence - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024)

Immunofluorescence analysis of U2OS cells using Alpha-tubulin (ubiquitous) chain antibody (AC024) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TUBA1B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TUBA1B. (Distance between topics and target gene indicate popularity.) TUBA1B

* Data provided by citexs.com, for reference only.

Publishing research using AC024? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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