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alpha Tubulin Monoclonal Antibody (AC012)

Publications (3) Datasheet

ABclonal:Western blot - alpha Tubulin Monoclonal Antibody (AC012)
ABclonal:Immunofluorescence - alpha Tubulin Monoclonal Antibody (AC012)

Overview

Product namealpha Tubulin Monoclonal Antibody
Catalog No.AC012
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant protein of human TUBA1B
SequenceEmail for sequence
Gene ID7277
Swiss protP68366
SynonymsTUBA4A; ALS22; H2-ALPHA; TUBA1; tubulin alpha 4a
Calculated MW48kDa/49kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:5000 - 1:15000
IHC 1:200 - 1:500
IF 1:100 - 1:200
IP 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, 293T, HeLa, Jurkat, Mouse spleen, Mouse thymus, Mouse brain, Rat brain, Rat kidney
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Rat, Human)

ABclonal:Western blot - alpha Tubulin Monoclonal Antibody (AC012) }

Western blot - alpha Tubulin Monoclonal Antibody (AC012)

Western blot analysis of extracts of various cell lines, using alpha Tubulin antibody (AC012) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - alpha Tubulin Monoclonal Antibody (AC012) }

Immunofluorescence - alpha Tubulin Monoclonal Antibody (AC012)

Immunofluorescence analysis of HeLa cells using TUBA4A antibody (AC012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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