Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZO-1 Rabbit pAb (A0659)

Reviews (2) Publications (90) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - ZO-1 Rabbit pAb (A0659)

Western blot analysis of lysates from Rat lung, using ZO-1 Rabbit pAb (A0659) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.

ABclonal:Immunofluorescence - ZO-1 Rabbit pAb (A0659)

Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit pAb (A0659) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for ZO-1 Rabbit pAb(A0659)
Review
ABclonal: review for ZO-1 Rabbit pAb(A0659)

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Overview

Product name ZO-1 Rabbit pAb
Catalog No. A0659
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 18-273 of human TJP1 (NP_003248.3).
Sequence IWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPD
Gene ID 7082
Swiss prot Q07157
Synonyms ZO-1
Calculated MW 195kDa
Observed MW 195kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Rat lung
Cellular location Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, gap junction, tight junction
Customer validation

(mouse sertoli cell, Mouse embryonal fibroblast cell)

WB (Homo sapiens, Mus musculus, Sus scrofa, Gallus gallus, Anatinae, Rattus norvegicus, mmu, Canis lupus familiaris, Horabagrus nigricollaris)

IF (Rattus norvegicus, Mus musculus, Homo sapiens, Cyprinus carpio, Sus scrofa, Epinephelus coioides, Ovis aries)

IHC (grass carp, Mus musculus, Rattus norvegicus, Homo sapiens, Horabagrus nigricollaris)

ICH (Rattus norvegicus)

ELISA (Rattus norvegicus, Homo sapiens, Mus musculus)

RT-PCR (Mus musculus)

Documents

Certificate of Compliance

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Lot number

ZO-1 Rabbit pAb images

ABclonal:Western blot - ZO-1 Rabbit pAb (A0659)}

Western blot - ZO-1 Rabbit pAb (A0659)

Western blot analysis of lysates from Rat lung, using ZO-1 Rabbit pAb (A0659) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.
ABclonal:Immunofluorescence - ZO-1 Rabbit pAb (A0659)}

Immunofluorescence - ZO-1 Rabbit pAb (A0659)

Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit pAb (A0659) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on TJP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TJP1. (Distance between topics and target gene indicate popularity.) TJP1

* Data provided by citexs.com, for reference only.

Publishing research using A0659? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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