Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZMYND8 Rabbit pAb (A8737)

Tested applications:WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human

Western blot analysis of extracts of various cell lines, using ZMYND8 antibody (A8737) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name	ZMYND8 Rabbit pAb
Catalog No.	A8737
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1).
Sequence	LELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD
Gene ID	23613
Swiss prot	Q9ULU4
Synonyms	ZMYND8; PRKCBP1; PRO2893; RACK7
Calculated MW	66kDa/82kDa/109-138kDa
Observed MW	165kDa

Applications

Reactivity	Human
Tested applications	WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:1000 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application key	Western blotting
Positive samples	U-87 MG, LO2, MCF-7, A375
Cellular location	cytoplasm, Golgi apparatus, nucleoplasm, nucleus

Research Area

ZMYND8 Rabbit pAb images

Western blot - ZMYND8 Rabbit pAb (A8737)

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on ZMYND8. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to ZMYND8. (Distance between topics and target gene indicate popularity.) ZMYND8

* Data provided by citexs.com, for reference only.