Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZMYND8 Rabbit pAb (A8737)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human

ABclonal:Western blot - ZMYND8 Rabbit pAb (A8737)

Western blot analysis of extracts of various cell lines, using ZMYND8 antibody (A8737) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameZMYND8 Rabbit pAb
Catalog No.A8737
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1).
SequenceLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD
Gene ID23613
Swiss protQ9ULU4
SynonymsZMYND8; PRKCBP1; PRO2893; RACK7
Calculated MW66kDa/82kDa/109-138kDa
Observed MW165kDa

Applications

ReactivityHuman
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesU-87 MG, LO2, MCF-7, A375
Cellular locationcytoplasm, Golgi apparatus, nucleoplasm, nucleus

Research Area

ZMYND8 Rabbit pAb images

ABclonal:Western blot - ZMYND8 Rabbit pAb (A8737)}

Western blot - ZMYND8 Rabbit pAb (A8737)

Western blot analysis of extracts of various cell lines, using ZMYND8 antibody (A8737) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZMYND8. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ZMYND8. (Distance between topics and target gene indicate popularity.) ZMYND8

* Data provided by citexs.com, for reference only.

Publishing research using A8737? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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