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ZMYM4 Rabbit pAb (A13812)

Datasheet

ABclonal:Western blot - ZMYM4 Polyclonal Antibody (A13812)

Overview

Product nameZMYM4 Rabbit pAb
Catalog No.A13812
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 35-230 of human ZMYM4 (NP_005086.2).
SequenceTEMSEDIDHNLTPTLDSMSYGMPNQTGSENSLLDEDDYFLNSGDLAGIPVVGSDNEDEQDFSSKDNLVSSIHTDDSLEVERRVTQHESDNENEIQIQNKLKKDFPKQFDQVSVFKSIRKDFSLVRENSKETFSGKEKNRDLTYEREKRLDKPHKDLDSRLKSSFFDKAANQVEETLHTHLPQTPETNFRDSSYPFA
Gene ID9202
Swiss protQ5VZL5
SynonymsZMYM4; CDIR; MYM; ZNF198L3; ZNF262
Calculated MW136kDa/163kDa/169kDa/172kDa
Observed MW240kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, HepG2, U-87MG, HeLa
Cellular location
    ABclonal:Western blot - ZMYM4 Polyclonal Antibody (A13812) }

    Western blot - ZMYM4 Polyclonal Antibody (A13812)

    Western blot analysis of extracts of various cell lines, using ZMYM4 Antibody (A13812) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

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