Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

ZEB1 Rabbit mAb (A21794)

Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)

Western blot analysis of extracts of HeLa cells, using ZEB1 antibody (A21794) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)

Western blot analysis of extracts of Mouse heart, using ZEB1 antibody (A21794) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

You may also interested in:

    Overview

    Product nameZEB1 Rabbit mAb
    Catalog No.A21794
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC53599

    Background

    This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 800-900 of human ZEB1 (NP_110378.3).
    SequenceINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTE
    Gene ID6935
    Swiss protP37275
    SynonymsBZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
    Calculated MW124kDa
    Observed MW200KDa

    Applications

    ReactivityHuman, Mouse
    Tested applicationsTesting results
    WBHumanMouse
    Recommended dilution
    • WB 1:1000 - 1:5000
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    
    Positive samplesHeLa, Mouse heart
    Cellular locationNucleus

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    ZEB1 Rabbit mAb images

    ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)}

    Western blot - ZEB1 Rabbit mAb (A21794)

    Western blot analysis of extracts of HeLa cells, using ZEB1 antibody (A21794) at1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)}

    Western blot - ZEB1 Rabbit mAb (A21794)

    Western blot analysis of extracts of Mouse heart, using ZEB1 antibody (A21794) at1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.

    Inquire About This Product

    Submit your question about A21794 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Related Targets

    Based on existing publications, the following genes are closely related to research on ZEB1. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to ZEB1. (Distance between topics and target gene indicate popularity.) ZEB1

    * Data provided by citexs.com, for reference only.

    Publishing research using A21794? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Select size and quantity
    Contact us to order