Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

ZEB1 Rabbit mAb (A21794)

Datasheet SDS COA

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse

ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)

Western blot analysis of extracts of HeLa cells, using ZEB1 antibody (A21794) at1:2200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)

Western blot analysis of extracts of Mouse heart, using ZEB1 antibody (A21794) at1:2200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product nameZEB1 Rabbit mAb
Catalog No.A21794
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53599

Background

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 800-900 of human ZEB1 (NP_110378.3).
SequenceINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTE
Gene ID6935
Swiss protP37275
SynonymsZEB1; AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
Calculated MW116kDa/122kDa/124kDa
Observed MW200KDa

Applications

ReactivityHuman, Mouse
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000 - 1:2200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, Mouse heart
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

Research Area

ZEB1 Rabbit mAb images

ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)}

Western blot - ZEB1 Rabbit mAb (A21794)

Western blot analysis of extracts of HeLa cells, using ZEB1 antibody (A21794) at1:2200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - ZEB1 Rabbit mAb (A21794)}

Western blot - ZEB1 Rabbit mAb (A21794)

Western blot analysis of extracts of Mouse heart, using ZEB1 antibody (A21794) at1:2200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

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