Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZCCHC7 Rabbit pAb (A18251)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - ZCCHC7 Rabbit pAb (A18251)

Western blot analysis of various lysates using ZCCHC7 Rabbit pAb (A18251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

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Overview

Product name ZCCHC7 Rabbit pAb
Catalog No. A18251
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables RNA binding activity. Located in cytosol and nucleolus.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human ZCCHC7 (NP_115602.2).
Sequence MMFGGYETIEAYEDDLYRDESSSELSVDSEVEFQLYSQIHYAQDLDDVIREEEHEEKNSGNSESSSSKPNQKKLIVLSDSEVIQLSDGSEVITLSDEDSIYRCKGKNVRVQAQENAHGLSSSLQSNELVDKKCKSDIEKPKSEERSGVIREVMIIEVSSSEEEESTISEGDNVESWMLLGCEVDDKDDDILLNLVGCENSVTEGEDGINWSISDKDIEAQIANNRTPGRWT
Gene ID 84186
Swiss prot Q8N3Z6
Synonyms AIR1; HSPC086; ZCCHC7
Calculated MW 63kDa
Observed MW 63kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse liver, Mouse heart, Rat brain
Cellular location cytosol, nucleolus
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

ZCCHC7 Rabbit pAb images

ABclonal:Western blot - ZCCHC7 Rabbit pAb (A18251)}

Western blot - ZCCHC7 Rabbit pAb (A18251)

Western blot analysis of various lysates using ZCCHC7 Rabbit pAb (A18251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZCCHC7. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ZCCHC7. (Distance between topics and target gene indicate popularity.) ZCCHC7

* Data provided by citexs.com, for reference only.

Publishing research using A18251? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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