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14-3-3 Theta Rabbit pAb (A2563)

Datasheet

ABclonal:Western blot - 14-3-3 Theta Rabbit pAb (A2563)

Western blot analysis of extracts of various cell lines, using 14-3-3 Theta antibody (A2563) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - 14-3-3 Theta Rabbit pAb (A2563)

Immunofluorescence analysis of HeLa cells using 14-3-3 Theta antibody (A2563). Blue: DAPI for nuclear staining.

Overview

Product name14-3-3 Theta Rabbit pAb
Catalog No.A2563
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human 14-3-3 Theta (NP_006817.1).
SequenceMEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN
Gene ID10971
Swiss protP27348
SynonymsYWHAQ; 14-3-3; 1C5; HS1
Calculated MW27kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSW480, BT-474, HeLa, Jurkat, Mouse liver, Mouse kidney, Mouse brain
Cellular locationCytoplasm
ABclonal:Western blot - 14-3-3 Theta Rabbit pAb (A2563) }

Western blot - 14-3-3 Theta Rabbit pAb (A2563)

Western blot analysis of extracts of various cell lines, using 14-3-3 Theta antibody (A2563) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - 14-3-3 Theta Rabbit pAb (A2563) }

Immunofluorescence - 14-3-3 Theta Rabbit pAb (A2563)

Immunofluorescence analysis of HeLa cells using 14-3-3 Theta antibody (A2563). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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