Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

YAP1 Rabbit pAb (A1001)

Publications (15) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - YAP1 Rabbit pAb (A1001)

Western blot analysis of various lysates using YAP1 Rabbit pAb (A1001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - YAP1 Rabbit pAb (A1001)

Immunohistochemistry analysis of YAP1 in paraffin-embedded human liver cancer using YAP1 Rabbit pAb (A1001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - YAP1 Rabbit pAb (A1001)

Immunohistochemistry analysis of YAP1 in paraffin-embedded human placenta using YAP1 Rabbit pAb (A1001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - YAP1 Rabbit pAb (A1001)

Immunohistochemistry analysis of YAP1 in paraffin-embedded rat brain using YAP1 Rabbit pAb (A1001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - YAP1 Rabbit pAb (A1001)

Immunofluorescence analysis of C6 cells using YAP1 Rabbit pAb (A1001) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - YAP1 Rabbit pAb (A1001)

Immunofluorescence analysis of NIH/3T3 cells using YAP1 Rabbit pAb (A1001) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name YAP1 Rabbit pAb
Catalog No. A1001
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
Sequence PTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL
Gene ID 10413
Swiss prot P46937
Synonyms YAP; YKI; COB1; YAP2; YAP-1; YAP65; YAP1
Calculated MW 54kDa
Observed MW 72kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples DU145, BT-474
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus)

IHC (Homo sapiens)

IF (Mus musculus, Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

YAP1 Rabbit pAb images

ABclonal:Western blot - YAP1 Rabbit pAb (A1001)}

Western blot - YAP1 Rabbit pAb (A1001)

Western blot analysis of various lysates using YAP1 Rabbit pAb (A1001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - YAP1 Rabbit pAb (A1001)}

Immunohistochemistry - YAP1 Rabbit pAb (A1001)

Immunohistochemistry analysis of YAP1 in paraffin-embedded human liver cancer using YAP1 Rabbit pAb (A1001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - YAP1 Rabbit pAb (A1001)}

Immunohistochemistry - YAP1 Rabbit pAb (A1001)

Immunohistochemistry analysis of YAP1 in paraffin-embedded human placenta using YAP1 Rabbit pAb (A1001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - YAP1 Rabbit pAb (A1001)}

Immunohistochemistry - YAP1 Rabbit pAb (A1001)

Immunohistochemistry analysis of YAP1 in paraffin-embedded rat brain using YAP1 Rabbit pAb (A1001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - YAP1 Rabbit pAb (A1001)}

Immunofluorescence - YAP1 Rabbit pAb (A1001)

Immunofluorescence analysis of C6 cells using YAP1 Rabbit pAb (A1001) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YAP1 Rabbit pAb (A1001)}

Immunofluorescence - YAP1 Rabbit pAb (A1001)

Immunofluorescence analysis of NIH/3T3 cells using YAP1 Rabbit pAb (A1001) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on YAP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to YAP1. (Distance between topics and target gene indicate popularity.) YAP1

* Data provided by citexs.com, for reference only.

Publishing research using A1001? Please let us know so that we can cite the reference in this datasheet.

Antibodies (13)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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