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XRCC6 Polyclonal Antibody (A7330)


ABclonal:Western blot - XRCC6 antibody (A7330)
ABclonal:Immunofluorescence - XRCC6 antibody (A7330)
ABclonal:Immunoprecipitation - 3ug XRCC6 antibody (A7330)


Product nameXRCC6 Polyclonal Antibody
Catalog No.A7330
Host speciesRabbit
Purification methodAffinity purification
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human XRCC6 (NP_001460.1).
Gene ID2547
Swiss protP12956
SynonymsCTC75; CTCBF; G22P1; KU70; ML8; TLAA
Calculated MW65kDa/69kDa
Observed MW70kDa
ReactivityHuman, Mouse, Monkey
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesCOS7, SW480, Mouse brain
Cellular locationChromosome, Nucleus
ABclonal:Western blot - XRCC6 antibody (A7330)}

Western blot - XRCC6 antibody (A7330)

Western blot analysis of extracts of various cell lines, using XRCC6 antibody (A7330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - XRCC6 antibody (A7330)}

Immunofluorescence - XRCC6 antibody (A7330)

Immunofluorescence analysis of A549 cells using XRCC6 antibody (A7330). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - 3ug XRCC6 antibody (A7330)}

Immunoprecipitation - 3ug XRCC6 antibody (A7330)

Immunoprecipitation analysis of 100ug extracts of SW480 cells using 3ug XRCC6 antibody (A7330). Western blot was performed from the immunoprecipitate using XRCC6 antibody (A7330) at a dilition of 1:1000.

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