Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

XRCC1 Mouse mAb (A0443)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - XRCC1 Mouse mAb (A0443)

Western blot analysis of various lysates using XRCC1 Mouse mAb (A0443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name XRCC1 Mouse mAb
Catalog No. A0443
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Immunogen information

Immunogen Recombinant protein of human XRCC1
Sequence Email for sequence
Gene ID 7515
Swiss prot P18887
Synonyms RCC; SCAR26; XRCC1
Calculated MW 69kDa
Observed MW 90kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Raji, K-562, HeLa, Jurkat, THP-1, MCF-7, NIH/3T3
Cellular location Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

IHC (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

XRCC1 Mouse mAb images

ABclonal:Western blot - XRCC1 Mouse mAb (A0443)}

Western blot - XRCC1 Mouse mAb (A0443)

Western blot analysis of various lysates using XRCC1 Mouse mAb (A0443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on XRCC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to XRCC1. (Distance between topics and target gene indicate popularity.) XRCC1

* Data provided by citexs.com, for reference only.

Publishing research using A0443? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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