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XPC Polyclonal Antibody (A8354)

Publication (1) Datasheet

ABclonal:Western blot - XPC antibody (A8354)
ABclonal:Immunohistochemistry - XPC antibody (A8354)

Overview

Product nameXPC Polyclonal Antibody
Catalog No.A8354
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 700-940 of human XPC (NP_004619.3).
SequenceGEVPYKMVKGFSNRARKARLAEPQLREENDLGLFGYWQTEEYQPPVAVDGKVPRNEFGNVYLFLPSMMPIGCVQLNLPNLHRVARKLDIDCVQAITGFDFHGGYSHPVTDGYIVCEEFKDVLLTAWENEQAVIERKEKEKKEKRALGNWKLLAKGLLIRERLKRRYGPKSEAAAPHTDAGGGLSSDEEEGTSSQAEAARILAASWPQNREDEEKQKLKGGPKKTKREKKAAASHLFPFEQL
Gene ID7508
Swiss protQ01831
Synonymsp125; RAD4; XP3; XPCC
Calculated MW15kDa/101kDa/105kDa
Observed MW130kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesLO2, HeLa, 293T, HT-1080, Jurkat
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human)

ABclonal:Western blot - XPC antibody (A8354)}

Western blot - XPC antibody (A8354)

Western blot analysis of extracts of various cell lines, using XPC antibody (A8354) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.
ABclonal:Immunohistochemistry - XPC antibody (A8354)}

Immunohistochemistry - XPC antibody (A8354)

Immunohistochemistry of paraffin-embedded human liver cancer using XPC antibody (A8354) at dilution of 1:100 (40x lens).

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