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Wnt5a Rabbit mAb (A19133)

Datasheet

ABclonal:Western blot - Wnt5a Rabbit mAb (A19133)
ABclonal:Immunohistochemistry - Wnt5a Rabbit mAb (A19133)

Overview

Product nameWnt5a Rabbit mAb
Catalog No.A19133
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
ImmunogenA synthesized peptide derived from human Wnt5a
SequenceEmail for sequence
Gene ID7474
Swiss protP41221
SynonymshWNT5A; Wnt5a; WNT5A
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, SKOV3
Cellular location
ABclonal:Western blot - Wnt5a Rabbit mAb (A19133) }

Western blot - Wnt5a Rabbit mAb (A19133)

Western blot analysis of extracts of various cell lines, using Wnt5a antibody (A19133) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Wnt5a Rabbit mAb (A19133) }

Immunohistochemistry - Wnt5a Rabbit mAb (A19133)

Immunohistochemistry of paraffin-embedded human lung cancer using Wnt5a Rabbit mAb (A19133) at dilution of 1:100 (40x lens).

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