Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

WDR77 Mouse mAb (A9921)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - WDR77 Mouse mAb (A9921)

Western blot analysis of extracts of various cell lines, using WDR77 antibody (A9921) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name WDR77 Mouse mAb
Catalog No. A9921
Host species Mouse
Purification method Affinity purification
Isotype IgG1, Kappa
CloneNo. AMC0495

Background

The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant protein of human WDR77
Sequence Email for sequence
Gene ID 79084
Swiss prot Q9BQA1
Synonyms p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50; WDR77
Calculated MW 37kDa
Observed MW 39kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples K-562, HeLa, 293T, Jurkat, HepG2, MCF7, SW480
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

WDR77 Mouse mAb images

ABclonal:Western blot - WDR77 Mouse mAb (A9921)}

Western blot - WDR77 Mouse mAb (A9921)

Western blot analysis of extracts of various cell lines, using WDR77 antibody (A9921) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on WDR77. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to WDR77. (Distance between topics and target gene indicate popularity.) WDR77

* Data provided by citexs.com, for reference only.

Publishing research using A9921? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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