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Vinculin Rabbit mAb (A2752)

Publications (2) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Vinculin Rabbit mAb (A2752)

Western blot analysis of extracts of various cell lines, using Vinculin Rabbit mAb (A2752) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Vinculin Rabbit mAb (A2752)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using Vinculin Rabbit mAb (A2752) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - Vinculin Rabbit mAb (A2752)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Vinculin antibody (A2752). Western blot was performed from the immunoprecipitate using Vinculin antibody (A2752) at a dilition of 1:1000.

Overview

Product nameVinculin Rabbit mAb
Catalog No.A2752
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).
SequenceDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM
Gene ID7414
Swiss protP18206
SynonymsCMD1W; CMH15; HEL114; MV; MVCL
Calculated MW125kDa
Observed MW124kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, HepG2, RD, Mouse liver, Mouse brain, Rat liver, Rat skeletal muscle, Rat brain
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion
Customer validation

WB(Homo sapiens, Oryctolagus cuniculus)

ABclonal:Western blot - Vinculin Rabbit mAb (A2752) }

Western blot - Vinculin Rabbit mAb (A2752)

Western blot analysis of extracts of various cell lines, using Vinculin Rabbit mAb (A2752) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Vinculin Rabbit mAb (A2752) }

Immunohistochemistry - Vinculin Rabbit mAb (A2752)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using Vinculin Rabbit mAb (A2752) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - Vinculin Rabbit mAb (A2752) }

Immunoprecipitation - Vinculin Rabbit mAb (A2752)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Vinculin antibody (A2752). Western blot was performed from the immunoprecipitate using Vinculin antibody (A2752) at a dilition of 1:1000.

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