Publications (9) Datasheet SDS COA
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates, using Vinculin antibody (A2752) at 1:250000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
| Product name | Vinculin Rabbit mAb |
|---|---|
| Catalog No. | A2752 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC51900 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1). |
|---|---|
| Sequence | DAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM |
| Gene ID | 7414 |
| Swiss prot | P18206 |
| Synonyms | MV; MVCL; CMD1W; CMH15; HEL114 |
| Calculated MW | 124kDa |
| Observed MW | 124kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | Testing results |
| WB | |
| IF | |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunofluorescence Immunoprecipitation |
| Positive samples | HeLa, HepG2, RD, Mouse liver, Mouse brain, Rat liver, Rat skeletal muscle, Rat brain |
| Cellular location | Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion |
| Customer validation | WB(Homo sapiens, Oryctolagus cuniculus, Anatinae, Mus musculus, Rattus norvegicus) |
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Based on existing publications, the following genes are closely related to research on VCL. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to VCL. (Distance between topics and target gene indicate popularity.) VCL
* Data provided by citexs.com, for reference only.
Publishing research using A2752? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
