Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Vinculin Rabbit mAb (A2752)

Publications (4) Datasheet SDS COA

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Vinculin Rabbit mAb (A2752)

Western blot analysis of extracts of various cell lines, using Vinculin Rabbit mAb (A2752) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Vinculin Rabbit mAb (A2752)

Confocal imaging of HeLa cells using Vinculin Rabbit mAb (A2752, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

ABclonal:Immunoprecipitation - Vinculin Rabbit mAb (A2752)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Vinculin antibody (A2752). Western blot was performed from the immunoprecipitate using Vinculin antibody (A2752) at a dilution of 1:1000.

Overview

Product nameVinculin Rabbit mAb
Catalog No.A2752
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51900

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).
SequenceDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM
Gene ID7414
Swiss protP18206
SynonymsCMD1W; CMH15; HEL114; MV; MVCL
Calculated MW125kDa
Observed MW124kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa, HepG2, RD, Mouse liver, Mouse brain, Rat liver, Rat skeletal muscle, Rat brain
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion
Customer validation

WB(Homo sapiens, Oryctolagus cuniculus, Anatinae)

Documents

Certificate of Compliance

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Research Area

Vinculin Rabbit mAb images

ABclonal:Western blot - Vinculin Rabbit mAb (A2752)}

Western blot - Vinculin Rabbit mAb (A2752)

Western blot analysis of extracts of various cell lines, using Vinculin Rabbit mAb (A2752) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Vinculin Rabbit mAb (A2752)}

Immunofluorescence - Vinculin Rabbit mAb (A2752)

Confocal imaging of HeLa cells using Vinculin Rabbit mAb (A2752, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
ABclonal:Immunoprecipitation - Vinculin Rabbit mAb (A2752)}

Immunoprecipitation - Vinculin Rabbit mAb (A2752)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Vinculin antibody (A2752). Western blot was performed from the immunoprecipitate using Vinculin antibody (A2752) at a dilution of 1:1000.

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