Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Vinculin Rabbit mAb (A2752)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Vinculin Rabbit mAb (A2752)

Western blot analysis of various lysates, using Vinculin antibody (A2752) at 1:250000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Vinculin Rabbit mAb (A2752)

Confocal imaging of HeLa cells using Vinculin Rabbit mAb (A2752, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

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Overview

Product name	Vinculin Rabbit mAb
Catalog No.	A2752
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51900

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).
Sequence	DAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM
Gene ID	7414
Swiss prot	P18206
Synonyms	MV; MVCL; CMD1W; CMH15; HEL114
Calculated MW	124kDa
Observed MW	124kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	Testing results
IF/ICC	Human
WB	HumanMouseRat
Recommended dilution	WB 1:10000 - 1:300000 IF/ICC 1:50 - 1:200 IP 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	HeLa, HepG2, RD, Mouse liver, Mouse brain, Rat liver, Rat skeletal muscle, Rat brain
Cellular location	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion
Customer validation	WB(Homo sapiens, Oryctolagus cuniculus, Anatinae, Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

Vinculin Rabbit mAb images

Western blot - Vinculin Rabbit mAb (A2752)

Immunofluorescence - Vinculin Rabbit mAb (A2752)

Confocal imaging of HeLa cells using Vinculin Rabbit mAb (A2752, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on VCL. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to VCL. (Distance between topics and target gene indicate popularity.) VCL

* Data provided by citexs.com, for reference only.

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Mol Cell

Application:

IF:

17.97

Species:

Homo sapiens

PMID:

34534457

Title:

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Bioact Mater

Application:

IF:

14.593

Species:

Oryctolagus cuniculus

PMID:

34901552

Title:

A functional PVA aerogel-based membrane obtaining sutureability through modified electrospinning technology and achieving promising anti-adhesion effect after cardiac surgery

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

36185211

Title:

The role of MARCH9 in colorectal cancer progression - PMC

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Animal nutrition (Zhongguo xu mu shou yi xue hui)

Application:

IF:

5.28

Species:

Anatinae

PMID:

35950191

Title:

Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks - PMC

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Medicine

Application:

IF:

1.81

Species:

Homo sapiens

PMID:

36401373

Title:

miR-195-3p/BDNF axis regulates hypoxic injury by targeting P-ERK1/2 expression - PMC

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Journal of assisted reproduction and genetics

Application:

IF:

3.35

Species:

Homo sapiens

PMID:

36469255

Title:

A novel heterozygous variant in PANX1 causes primary infertility due to oocyte death

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Biochimica et biophysica acta. Gene regulatory mechanisms

Application:

IF:

6.3

Species:

Mus musculus

PMID:

35217218

Title:

Differential requirements for different subfamilies of the mammalian SWI/SNF chromatin remodeling enzymes in myoblast differentiation

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

EBioMedicine

Application:

IF:

11.2

Species:

Homo sapiens

PMID:

36893588

Title:

Cytotoxic CD161-CD8+ TEMRA cells contribute to the pathogenesis of systemic lupus erythematosus - PMC

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Frontiers in molecular neuroscience

Application:

IF:

6.26

Species:

Rattus norvegicus

PMID:

36385763

Title:

Neuroinflammation in the medial prefrontal cortex exerts a crucial role in bone cancer pain - PMC

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Journal of genetics and genomics = Yi chuan xue bao

Application:

IF:

5.72

Species:

Homo sapiens

PMID:

37003352

Title:

Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Molecular carcinogenesis

Application:

IF:

5.13

Species:

Homo sapiens

PMID:

37036190

Title:

Targeting SMYD2 inhibits prostate cancer cell growth by regulating c‐Myc signaling

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Homo sapiens,Mus musculus

PMID:

37276643

Title:

Fluoxetine inactivates STAT3/NF-κB signaling and promotes sensitivity to cisplatin in bladder cancer

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Mus musculus

PMID:

37148740

Title:

NFATc1-mediated expression of SLC7A11 drives sensitivity to TXNRD1 inhibitors in osteoclast precursors

References for Vinculin Rabbit mAb (A2752)

Product:

Vinculin Rabbit mAb

Journal:

Clinical Medicine Insights. Oncology

Application:

IF:

1.79

Species:

Homo sapiens

PMID:

37435017

Title:

Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles - PMC