Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Vinculin Rabbit mAb (A2752)

Publications (9) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Vinculin Rabbit mAb (A2752)

Western blot analysis of various lysates, using Vinculin antibody (A2752) at 1:250000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Vinculin Rabbit mAb (A2752)

Confocal imaging of HeLa cells using Vinculin Rabbit mAb (A2752, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

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    Overview

    Product nameVinculin Rabbit mAb
    Catalog No.A2752
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC51900

    Background

    Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).
    SequenceDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM
    Gene ID7414
    Swiss protP18206
    SynonymsMV; MVCL; CMD1W; CMH15; HEL114
    Calculated MW124kDa
    Observed MW124kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsTesting results
    WBHumanMouseRat
    IFHuman
    Recommended dilution
    • WB 1:10000 - 1:300000
    • IF/ICC 1:50 - 1:200
    • IP 1:500 - 1:1000
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
    Positive samplesHeLa, HepG2, RD, Mouse liver, Mouse brain, Rat liver, Rat skeletal muscle, Rat brain
    Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion
    Customer validation

    WB(Homo sapiens, Oryctolagus cuniculus, Anatinae, Mus musculus, Rattus norvegicus)

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    Vinculin Rabbit mAb images

    ABclonal:Western blot - Vinculin Rabbit mAb (A2752)}

    Western blot - Vinculin Rabbit mAb (A2752)

    Western blot analysis of various lysates, using Vinculin antibody (A2752) at 1:250000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - Vinculin Rabbit mAb (A2752)}

    Immunofluorescence - Vinculin Rabbit mAb (A2752)

    Confocal imaging of HeLa cells using Vinculin Rabbit mAb (A2752, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on VCL. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to VCL. (Distance between topics and target gene indicate popularity.) VCL

    * Data provided by citexs.com, for reference only.

    Publishing research using A2752? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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