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Vimentin Polyclonal Antibody (A2584)

Reviews (3)Publications (4) Datasheet

ABclonal:Western blot - Vimentin Polyclonal Antibody (A2584)
ABclonal:Immunohistochemistry - Vimentin Polyclonal Antibody (A2584)
ABclonal:Immunohistochemistry - Vimentin Polyclonal Antibody (A2584)
ABclonal:Immunohistochemistry - Vimentin Polyclonal Antibody (A2584)
ABclonal:Immunofluorescence - Vimentin Polyclonal Antibody (A2584)
Review
ABclonal: review for Vimentin Polyclonal Antibody(A2584)
Review
ABclonal: review for Vimentin Polyclonal Antibody(A2584)
Review
ABclonal: review for Vimentin Polyclonal Antibody(A2584)

Overview

Product nameVimentin Polyclonal Antibody
Catalog No.A2584
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin (NP_003371.2).
SequenceMSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAE
Gene ID7431
Swiss protP08670
SynonymsVIM; CTRCT30; HEL113; vimentin
Calculated MW53kDa
Observed MW55kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, HeLa
Cellular locationCytoplasm
Customer validation

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IHC(Human, Mouse)

IF(Human)

WB(Human)

ABclonal:Western blot - Vimentin Polyclonal Antibody (A2584) }

Western blot - Vimentin Polyclonal Antibody (A2584)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A2584) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Vimentin Polyclonal Antibody (A2584) }

Immunohistochemistry - Vimentin Polyclonal Antibody (A2584)

Immunohistochemistry of paraffin-embedded human lung using Vimentin antibody (A2584) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Vimentin Polyclonal Antibody (A2584) }

Immunohistochemistry - Vimentin Polyclonal Antibody (A2584)

Immunohistochemistry of paraffin-embedded human lung cancer using Vimentin antibody (A2584) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Vimentin Polyclonal Antibody (A2584) }

Immunohistochemistry - Vimentin Polyclonal Antibody (A2584)

Immunohistochemistry of paraffin-embedded mouse lung using Vimentin antibody (A2584) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Vimentin Polyclonal Antibody (A2584) }

Immunofluorescence - Vimentin Polyclonal Antibody (A2584)

Immunofluorescence analysis of U2OS cells using Vimentin antibody (A2584) at dilution of 1:100. Blue: DAPI for nuclear staining.

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