Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VPS28 Rabbit pAb (A9104)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - VPS28 Rabbit pAb (A9104)

Western blot analysis of various lysates using VPS28 Rabbit pAb (A9104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name VPS28 Rabbit pAb
Catalog No. A9104
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human VPS28 (NP_057292.1).
Sequence MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA
Gene ID 51160
Swiss prot Q9UK41
Synonyms VPS28
Calculated MW 25kDa
Observed MW 25kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples A-549, U-251MG, 293T, HepG2, Mouse brain, Mouse kidney, Mouse liver, Mouse spleen, Rat brain
Cellular location Cell membrane, Late endosome membrane, Peripheral membrane protein

Documents

Certificate of Compliance

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VPS28 Rabbit pAb images

ABclonal:Western blot - VPS28 Rabbit pAb (A9104)}

Western blot - VPS28 Rabbit pAb (A9104)

Western blot analysis of various lysates using VPS28 Rabbit pAb (A9104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on VPS28. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VPS28. (Distance between topics and target gene indicate popularity.) VPS28

* Data provided by citexs.com, for reference only.

Publishing research using A9104? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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