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VHL Rabbit pAb (A0377)

Reviews (2)Publications (4) Datasheet

ABclonal:Western blot - VHL Rabbit pAb (A0377)

Western blot analysis of extracts of various cell lines, using VHL antibody (A0377) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - VHL Rabbit pAb (A0377)

Immunohistochemistry of paraffin-embedded rat ovary using VHL antibody (A0377) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - VHL Rabbit pAb (A0377)

Immunohistochemistry of paraffin-embedded human tonsil using VHL antibody (A0377) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - VHL Rabbit pAb (A0377)

Immunohistochemistry of paraffin-embedded mouse spleen using VHL antibody (A0377) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - VHL Rabbit pAb (A0377)

Immunofluorescence analysis of U-251 MG cells using VHL Polyclonal Antibody (A0377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for VHL Rabbit pAb(A0377)
Review
ABclonal: review for VHL Rabbit pAb(A0377)

Overview

Product nameVHL Rabbit pAb
Catalog No.A0377
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human VHL (NP_937799.1).
SequenceMPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD
Gene ID7428
Swiss protP40337
SynonymsVHL; HRCA1; RCA1; VHL1; pVHL; PVHL
Calculated MW18kDa/19kDa/24kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse brain, Rat brain
Cellular locationCytoplasm, Membrane, Nucleus, Nucleus, Peripheral membrane protein
Customer validation

(human kidney cancer cells, LNCaP)

WB(Homo sapiens, Danio rerio)

IF(Homo sapiens)

ABclonal:Western blot - VHL Rabbit pAb (A0377) }

Western blot - VHL Rabbit pAb (A0377)

Western blot analysis of extracts of various cell lines, using VHL antibody (A0377) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - VHL Rabbit pAb (A0377) }

Immunohistochemistry - VHL Rabbit pAb (A0377)

Immunohistochemistry of paraffin-embedded rat ovary using VHL antibody (A0377) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - VHL Rabbit pAb (A0377) }

Immunohistochemistry - VHL Rabbit pAb (A0377)

Immunohistochemistry of paraffin-embedded human tonsil using VHL antibody (A0377) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - VHL Rabbit pAb (A0377) }

Immunohistochemistry - VHL Rabbit pAb (A0377)

Immunohistochemistry of paraffin-embedded mouse spleen using VHL antibody (A0377) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - VHL Rabbit pAb (A0377) }

Immunofluorescence - VHL Rabbit pAb (A0377)

Immunofluorescence analysis of U-251 MG cells using VHL Polyclonal Antibody (A0377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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