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VEGF Receptor 2 Rabbit pAb (A5609)

Reviews (3)Publications (5) Datasheet

ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (A5609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (A5609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Review
ABclonal: review for VEGF Receptor 2 Rabbit pAb(A5609)
Review
ABclonal: review for VEGF Receptor 2 Rabbit pAb(A5609)
Review
ABclonal: review for VEGF Receptor 2 Rabbit pAb(A5609)

Overview

Product nameVEGF Receptor 2 Rabbit pAb
Catalog No.A5609
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1150-1250 of human VEGF Receptor 2 (NP_002244.1).
SequenceRPTFSELVEHLGNLLQANAQQDGKDYIVLPISETLSMEEDSGLSLPTSPVSCMEEEEVCDPKFHYDNTAGISQYLQNSKRKSRPVSVKTFEDIPLEEPEVK
Gene ID3791
Swiss protP35968
SynonymsCD309; FLK1; VEGFR; VEGFR2; VEGF Receptor 2; KDR
Calculated MW75kDa/79kDa/151kDa
Observed MW210KDa/230KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesECV-304, Mouse skeletal muscle, Mouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Early endosome, Endoplasmic reticulum, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation

(Eoma cell, HUVEC cells)

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609) }

Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (A5609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - VEGF Receptor 2 Rabbit pAb (A5609) }

Western blot - VEGF Receptor 2 Rabbit pAb (A5609)

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (A5609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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