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VEGF Rabbit pAb (A5708)

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ABclonal:Western blot - VEGF Rabbit pAb (A5708)

Western blot analysis of extracts of various cell lines, using VEGF antibody (A5708) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Review
ABclonal: review for VEGF Rabbit pAb(A5708)
Review
ABclonal: review for VEGF Rabbit pAb(A5708)

Overview

Product nameVEGF Rabbit pAb
Catalog No.A5708
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human VEGF (NP_001165099.1).
SequenceMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR
Gene ID7422
Swiss protP15692
SynonymsVEGFA; MVCD1; VEGF; VPF; L VEGFA; VEGF A
Calculated MW15-27kDa/34-45kDa
Observed MW38kDa/27KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesA-549, BT-474, Mouse heart, Mouse kidney, Mouse gastrocnemius muscle, Rat lung, Rat kidney
Cellular locationSecreted
Customer validation

(HUVEC cells, H460)

WB(Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus)

IHC(Homo sapiens)

IHC(Gallus gallus)

IF(Gallus gallus)

ABclonal:Western blot - VEGF Rabbit pAb (A5708) }

Western blot - VEGF Rabbit pAb (A5708)

Western blot analysis of extracts of various cell lines, using VEGF antibody (A5708) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

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