Products > Catalog Antibodies > Polyclonal Antibodies

VEGF Rabbit pAb (A12303)

Review (1)Publications (14) Datasheet

ABclonal:Western blot - VEGF Rabbit pAb (A12303)

Western blot analysis of extracts of various cell lines, using VEGF antibody (A12303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303)

Immunohistochemistry of paraffin-embedded Human esophagus cancer using VEGF antibody (A12303) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303)

Immunohistochemistry of paraffin-embedded Human lung cancer using VEGF antibody (A12303) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - VEGF Rabbit pAb (A12303)

Immunofluorescence analysis of HUVEC cells using VEGFA antibody (A12303) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for VEGF Rabbit pAb(A12303)

Overview

Product nameVEGF Rabbit pAb
Catalog No.A12303
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-126AA of human VEGF (NP_001165099.1).
SequenceAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN
Gene ID7422
Swiss protP15692
SynonymsVEGFA; MVCD1; VEGF; VPF; L VEGFA; VEGF A
Calculated MW15-27kDa/34-45kDa
Observed MW16KDa/34KDa/45KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, A-549, HeLa, Mouse liver
Cellular locationSecreted
Customer validation

(Mouse bone tissue)

WB(Neovison vison, Mus musculus, Oryctolagus cuniculus, Homo sapiens, Gallus gallus, Rattus norvegicus)

IF(Rattus norvegicus, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - VEGF Rabbit pAb (A12303) }

Western blot - VEGF Rabbit pAb (A12303)

Western blot analysis of extracts of various cell lines, using VEGF antibody (A12303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303) }

Immunohistochemistry - VEGF Rabbit pAb (A12303)

Immunohistochemistry of paraffin-embedded Human esophagus cancer using VEGF antibody (A12303) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303) }

Immunohistochemistry - VEGF Rabbit pAb (A12303)

Immunohistochemistry of paraffin-embedded Human lung cancer using VEGF antibody (A12303) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - VEGF Rabbit pAb (A12303) }

Immunofluorescence - VEGF Rabbit pAb (A12303)

Immunofluorescence analysis of HUVEC cells using VEGFA antibody (A12303) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A12303

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A12303 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A12303? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order