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VE Cadherin Rabbit pAb (A0734)

Review (1)Publications (4) Datasheet

ABclonal:Western blot - VE Cadherin Rabbit pAb (A0734)

Western blot analysis of extracts of various cell lines, using VE Cadherin antibody (A0734) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for VE Cadherin Rabbit pAb(A0734)

Overview

Product nameVE Cadherin Rabbit pAb
Catalog No.A0734
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 259-358 of human VE Cadherin (NP_001786.2).
SequenceTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQ
Gene ID1003
Swiss protP33151
SynonymsCDH5; 7B4; CD144; cadherin-5
Calculated MW74kDa/87kDa
Observed MW120-140KDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse lung, Rat lung
Cellular locationCell junction, Cell membrane, Single-pass type I membrane protein
Customer validation

(HUVEC)

WB(Bos taurus , Mus musculus, Homo sapiens, Sus scrofa)

IF(Homo sapiens)

ABclonal:Western blot - VE Cadherin Rabbit pAb (A0734) }

Western blot - VE Cadherin Rabbit pAb (A0734)

Western blot analysis of extracts of various cell lines, using VE Cadherin antibody (A0734) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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