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VCAM1 Rabbit pAb (A0279)

Publication (6) Datasheet

ABclonal:Western blot - VCAM1 Polyclonal Antibody (A0279)

Overview

Product nameVCAM1 Rabbit pAb
Catalog No.A0279
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1).
SequenceNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVEL
Gene ID7412
Swiss protP19320
SynonymsCD106; INCAM-100; VCAM1
Calculated MW71kDa/74kDa/81kDa
Observed MW103kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:3000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, HeLa
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

WB(Mouse, Rat, Human, Duck)

ABclonal:Western blot - VCAM1 Polyclonal Antibody (A0279) }

Western blot - VCAM1 Polyclonal Antibody (A0279)

Western blot analysis of extracts of various cell lines, using VCAM1 Antibody (A0279) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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