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Ubiquitin Rabbit pAb (A0162)

Publications (9) Datasheet

ABclonal:Western blot - Ubiquitin Rabbit pAb (A0162)

Overview

Product nameUbiquitin Rabbit pAb
Catalog No.A0162
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide of human Ubiquitin
SequenceEmail for sequence
Gene ID7314
Swiss protP0CG47
SynonymsHEL-S-50; Ubiquitin; UBB
Calculated MW25kDa
Observed MW26KDa/37KDa/60KDa/80KDa/115KDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, Mouse kidney
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Crassostrea gigas, Mus musculus)

IF(Brachyura)

ABclonal:Western blot - Ubiquitin Rabbit pAb (A0162) }

Western blot - Ubiquitin Rabbit pAb (A0162)

Western blot analysis of extracts of various cell lines, using Ubiquitin pAb (A0162) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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