Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

USP21 Rabbit pAb (A16663)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - USP21 Rabbit pAb (A16663)

Western blot analysis of extracts of Mouse brain, using USP21 antibody (A16663) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - USP21 Rabbit pAb (A16663)

Immunofluorescence analysis of HeLa cells using USP21 Rabbit pAb (A16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - USP21 Rabbit pAb (A16663)

Immunofluorescence analysis of PC-12 cells using USP21 Rabbit pAb (A16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - USP21 Rabbit pAb (A16663)

Immunofluorescence analysis of RAW264.7 cells using USP21 Rabbit pAb (A16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name USP21 Rabbit pAb
Catalog No. A16663
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP21 (NP_036607.3).
Sequence MPQASEHRLGRTREPPVN
Gene ID 27005
Swiss prot Q9UK80
Synonyms USP16; USP23; USP21
Calculated MW 63kDa
Observed MW 63kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse brain
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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USP21 Rabbit pAb images

ABclonal:Western blot - USP21 Rabbit pAb (A16663)}

Western blot - USP21 Rabbit pAb (A16663)

Western blot analysis of extracts of Mouse brain, using USP21 antibody (A16663) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - USP21 Rabbit pAb (A16663)}

Immunofluorescence - USP21 Rabbit pAb (A16663)

Immunofluorescence analysis of HeLa cells using USP21 Rabbit pAb (A16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - USP21 Rabbit pAb (A16663)}

Immunofluorescence - USP21 Rabbit pAb (A16663)

Immunofluorescence analysis of PC-12 cells using USP21 Rabbit pAb (A16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - USP21 Rabbit pAb (A16663)}

Immunofluorescence - USP21 Rabbit pAb (A16663)

Immunofluorescence analysis of RAW264.7 cells using USP21 Rabbit pAb (A16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on USP21. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to USP21. (Distance between topics and target gene indicate popularity.) USP21

* Data provided by citexs.com, for reference only.

Publishing research using A16663? Please let us know so that we can cite the reference in this datasheet.

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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