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USO1 Rabbit pAb (A16079)

Datasheet

ABclonal:Western blot - USO1 Polyclonal Antibody (A16079)

Western blot analysis of extracts of various cell lines, using USO1 antibody (A16079) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - USO1 Polyclonal Antibody (A16079)

Immunofluorescence analysis of HeLa cells using USO1 antibody (A16079) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - USO1 Polyclonal Antibody (A16079)

Confocal immunofluorescence analysis of Hela cells using USO1 antibody (A16079) at dilution of 1:200. Blue: DAPI for nuclear staining.

Overview

Product nameUSO1 Rabbit pAb
Catalog No.A16079
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 663-962 of human USO1 (NP_003706.2).
SequenceDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI
Gene ID8615
Swiss protO60763
SynonymsUSO1; P115; TAP; VDP
Calculated MW107kDa/109kDa
Observed MW108kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, Raji, SGC7901
Cellular locationCytoplasm, Golgi apparatus membrane, Peripheral membrane protein, cytosol
ABclonal:Western blot - USO1 Polyclonal Antibody (A16079) }

Western blot - USO1 Polyclonal Antibody (A16079)

Western blot analysis of extracts of various cell lines, using USO1 antibody (A16079) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - USO1 Polyclonal Antibody (A16079) }

Immunofluorescence - USO1 Polyclonal Antibody (A16079)

Immunofluorescence analysis of HeLa cells using USO1 antibody (A16079) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - USO1 Polyclonal Antibody (A16079) }

Immunofluorescence - USO1 Polyclonal Antibody (A16079)

Confocal immunofluorescence analysis of Hela cells using USO1 antibody (A16079) at dilution of 1:200. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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