Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

UPB1 Rabbit pAb (A15452)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - UPB1 Rabbit pAb (A15452)

Western blot analysis of various lysates using UPB1 Rabbit pAb (A15452) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name UPB1 Rabbit pAb
Catalog No. A15452
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that belongs to the CN hydrolase family. Beta-ureidopropionase catalyzes the last step in the pyrimidine degradation pathway. The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta-aminoisobutyric acid, respectively. UP deficiencies are associated with N-carbamyl-beta-amino aciduria and may lead to abnormalities in neurological activity.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human UPB1 (NP_057411.1).
Sequence MAGAEWKSLEECLEKHLPLPDLQEVKRVLYGKELRKLDLPREAFEAASREDFELQGYAFEAAEEQLRRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNLGHPVFQTQFGRIAVNICYGRHHPL
Gene ID 51733
Swiss prot Q9UBR1
Synonyms BUP1; UPB1
Calculated MW 43kDa
Observed MW 43kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse kidney, Mouse liver, Rat kidney
Cellular location Cytoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

UPB1 Rabbit pAb images

ABclonal:Western blot - UPB1 Rabbit pAb (A15452)}

Western blot - UPB1 Rabbit pAb (A15452)

Western blot analysis of various lysates using UPB1 Rabbit pAb (A15452) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on UPB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to UPB1. (Distance between topics and target gene indicate popularity.) UPB1

* Data provided by citexs.com, for reference only.

Publishing research using A15452? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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