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ULk1 Rabbit pAb (A8529)

Publications (3) Datasheet

ABclonal:Western blot - ULk1 Polyclonal Antibody (A8529)
ABclonal:Immunohistochemistry - ULk1 Polyclonal Antibody (A8529)
ABclonal:Immunohistochemistry - ULk1 Polyclonal Antibody (A8529)
ABclonal:Immunofluorescence - ULk1 Polyclonal Antibody (A8529)

Overview

Product nameULk1 Rabbit pAb
Catalog No.A8529
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 400-499 of human ULk1 (NP_003556.1).
SequenceRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGG
Gene ID8408
Swiss protO75385
SynonymsULK1; ATG1; ATG1A; UNC51; Unc51.1; hATG1; ULk1
Calculated MW112kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HeLa
Cellular locationCytoplasm, Preautophagosomal structure, cytosol
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - ULk1 Polyclonal Antibody (A8529) }

Western blot - ULk1 Polyclonal Antibody (A8529)

Western blot analysis of extracts of various cell lines, using ULK1 antibody (A8529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - ULk1 Polyclonal Antibody (A8529) }

Immunohistochemistry - ULk1 Polyclonal Antibody (A8529)

Immunohistochemistry of paraffin-embedded mouse liver using ULk1 antibody (A8529) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ULk1 Polyclonal Antibody (A8529) }

Immunohistochemistry - ULk1 Polyclonal Antibody (A8529)

Immunohistochemistry of paraffin-embedded mouse heart using ULk1 antibody (A8529) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - ULk1 Polyclonal Antibody (A8529) }

Immunofluorescence - ULk1 Polyclonal Antibody (A8529)

Immunofluorescence analysis of L929 cells using ULK1 antibody (A8529) at dilution of 1:100. Blue: DAPI for nuclear staining.

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