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ULK1 Rabbit pAb (A8529)

Publications (7) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - ULK1 Rabbit pAb (A8529)

Western blot analysis of extracts of Mouse brain, using ULK1 antibody (A8529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

ABclonal:Western blot - ULK1 Rabbit pAb (A8529)

Western blot analysis of extracts of Rat brain, using ULK1 antibody (A8529) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - ULK1 Rabbit pAb (A8529)

Immunofluorescence analysis of L929 cells using ULK1 antibody (A8529) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameULK1 Rabbit pAb
Catalog No.A8529
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1 via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. Phosphorylates FLCN, promoting autophagy. Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 262-280 of human ULK1 (NP_003556.2).
SequencePPSGS
Gene ID8408
Swiss protO75385
SynonymsULK1; ATG1; ATG1A; UNC51; Unc51.1; hATG1; ULk1
Calculated MW112kDa
Observed MW150KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
  • IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesRD, 293T, Mouse brain, Rat brain
Cellular locationCytoplasm, Preautophagosomal structure, cytosol
Customer validation

WB(Homo sapiens, Mus musculus, Spodoptera frugiperda)

IHC(Mus musculus)

ABclonal:Western blot - ULK1 Rabbit pAb (A8529)}

Western blot - ULK1 Rabbit pAb (A8529)

Western blot analysis of extracts of Mouse brain, using ULK1 antibody (A8529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.
ABclonal:Western blot - ULK1 Rabbit pAb (A8529)}

Western blot - ULK1 Rabbit pAb (A8529)

Western blot analysis of extracts of Rat brain, using ULK1 antibody (A8529) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - ULK1 Rabbit pAb (A8529)}

Immunofluorescence - ULK1 Rabbit pAb (A8529)

Immunofluorescence analysis of L929 cells using ULK1 antibody (A8529) at dilution of 1:100. Blue: DAPI for nuclear staining.

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